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| Costal-Oliveira,Fernanda; Guerra-Duarte,Clara; Oliveira,Maira Souza; Castro,Karen Larissa Pereira de; Lopes-de-Sousa,Leticia; Lara,Aline; Gomes,Enéas Ricardo de Morais; Bonilla,Cesar; Guatimosim,Sílvia; Melo,Marília Martins; Chávez-Olórtegui,Carlos. |
| Abstract Background Hadruroides lunatus is the most abundant scorpion species in the Peruvian central coast, where most of the accidents involving humans are registered. In spite of its prevalence, there are only very few studies on H. lunatus envenomation. The aim of the present study was to analyze the cardiorespiratory alterations caused by H. lunatus envenomation in rodents. Methods Wistar rats injected with H. lunatus scorpion venom were submitted to electrocardiography. After euthanasia, rat lungs were collected and histopathologically analyzed. Mouse cardiomyocytes were used to perform immunofluorescence and calcium transient assays. Data were analyzed by ANOVA or Student’s t-test. The significance level was set at p< 0.05. Results It was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hadruroides lunatus venom; Cardiorespiratory alterations; Electrocardiography; Immunofluorescence; Calcium transient. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100301 |
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| Severo,J.S.; Aoki,V.; Santana,A.E.; Mantovani,M.M.; Michalany,N.S.; Larsson Junior,C.E.; Larsson,C.E.. |
| ABSTRACT Pemphigus foliaceus (PF) is the most common autoimmune skin disease in dogs. It is characterized by pustules, erosions, and crusts which occur due to the presence of autoantibodies that target intercellular adhesion. Histopathological examination is considered the gold standard pattern in the diagnosis, but may sometimes be inconclusive, especially when the characteristic findings are not identified. New diagnostic tests are continuously being developed and immunofluorescence assays, could be a valuable alternative diagnostic tool. This study aimed to evaluate the applicability of direct and indirect immunofluorescence (DIF and IIF) tests for the diagnosis of canine PF. Twenty eight dogs were divided into two groups: Group I with 14 dogs with PF... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Immunofluorescence; DIF; IIF; Pemphigus foliaceus. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000300649 |
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| Soler,Maritza C; Molina,Jessica L; Díaz,Hugo A; Pinto,Vivian C; Barrios,Yasenka L; He,Kan; Roller,Marc; Weinstein-Oppenheimer,Caroline R. |
| Cimicifuga foetida, an Asian Cimicifuga species, has been employed as a cooling and detoxification agent in traditional Chinese medicine since ancient times. For this herb, two cycloartane triterpene glycosides isolated from the rhizomes have demonstrated cytotoxicity on rat tumor and human cancer cell lines. Since human Hsp27 is increased in various human cancers and exhibits cytoprotective activity that affects tumorigenesis and the susceptibility of tumours to cancer treatment, the purpose of this research was to study the expression of this protein in MCF-7 breast cancer cells. To accomplish this aim, MCF-7 cells were exposed to different concentrations of Cimicifuga foetida extract showing a reduction in cell number measured by the sulforhodamine... |
| Tipo: Journal article |
Palavras-chave: Cimicifuga foetida; Cytotoxicity; Hsp-27; Immunofluorescence; MCF-7 cells; RT-PCR. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000300005 |
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| Al-Younes,Hesham M.; Paldanius,Mika. |
| The bacterium Simkania negevensis is a germ associated with respiratory diseases. This study aims at estimating the prevalence of Simkania in the Jordanian population. Serum samples from 664 Jordanian males and females, aged 2 to 86 years were collected. IgG and IgM Simkania-specific antibodies were detected using an indirect immunofluorescence test. Seropositivity titers for IgG and IgM were defined as 1:8 and 1:10, respectively. The overall prevalence of IgG antibody in all examined Jordanian nationals was 58.4%. IgG seropositivity was low in children under the age of 10 years (34.2%), and increased rapidly with age and ranged between 49.4% and 72%. Simkania-specific IgM was detected in 24.8% of subjects. IgM prevalence in children under 10 years was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Jordan; Immunofluorescence; Prevalence; Simkania. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400038 |
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| Bosso,Patrícia Alves Ramos; Candeias,João Manuel Grisi; Paduan,Karina dos Santos; Ricchetti,Sandra Mara Queiroz; Miranda,Antero Frederico Macedo de; Rugolo,Lígia Maria Suppo de Souza; Durigon,Edison Luiz; Ventura,Armando Morais. |
| Human Respiratory Syncytial Virus (HRSV) is recognized as the single most important pathogen responsible for acute viral infection of the lower respiratory tract in young children. In this study, 84 samples from children under two years of age presenting acute respiratory symptoms were obtained from September 2000 to November 2001. Analysis by immunofluorescence and reverse-transcription followed by PCR, revealed that 18% (15/84) of all the samples were positive for HRSV, in which 80% (12/15) of the cases was observed in children under six months of age and the subgroups A and B co-circulated in the studied period. These are the first data obtained for Botucatu city and seasonality is evident by the higher circulation of the virus between May and July. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Human Respiratory Syncytial Virus (HRSV); RT-PCR; Immunofluorescence; Seasonality. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000300015 |
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| Huo,Shengdong; Yuan,Qijian; Zhang,Taojie; Liu,Junlin; Abudureyimu,Ayimuguli; Yang,Jutian. |
| ABSTRACTThe objective of this study was to investigate mRNA by real-time PCR and protein expression by immunofluorescence of the estradiol receptors (ER) in the pineal gland, hypothalamus, pituitary gland, and gonads of yaks (Bos grunniens). The analysis showed that the level of expression of ER mRNA was greater in the pituitary gland tissue than in other glands during estrus. Immunofluorescence analyses showed that ER proteins were located in the pineal cells, synaptic ribbon, and synaptic spherules of the pineal gland. In the hypothalamus, ER proteins were located in the magnocellular and parvocellular neurons. The ER proteins were located in acidophilic cells and basophilic cells in the pituitary gland. In the ovary, ER proteins were present in the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Immunofluorescence; Real-time PCR. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982015001000350 |
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| Huo,Sheng-dong; Zhang,Tao-jie; Abudureyimu,Ayimuguli; Liu,Jun-lin; Zhang,Guo-hua; Ma,Zhong-ren. |
| ABSTRACT To demonstrate the role of gonadotropin-releasing hormone (GnRH) in yaks (Bos grunniens), we characterized the expression of gonadotropin-releasing hormone receptor (GnRHR) mRNA and protein. The level of GnRHR mRNA in the hypothalamus was higher than that in the pineal gland, pituitary gland, and ovary during estrus. Immunofluorescence analysis showed that GnRHR was expressed in the pinealocyte, synaptic ribbon, and synaptic spherules of the pineal gland and that melatonin interacts with GnRHR via nerve fibers. In the hypothalamus, GnRHR was expressed in the magnocellular neurons and parvocellular neurons. In the pituitary gland, GnRHR was expressed in acidophilic cells and basophilic cells. In the ovary, GnRHR was present in the ovarian follicle... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bos grunniens; Gonadotropin-releasing hormone receptor; Immunofluorescence; Real-time PCR. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100401 |
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| Lorin, N; Boulo, Viviane; Bodinier, C; Charmantier, G. |
| This study combines a cellular and molecular analysis of the Na+/K+/2Cl(-) cotransporter (NKCC) to determine the osmoregulatory role of this protein in different tissues during the ontogeny of the sea bass. We have characterized the complete sequence of the NKCC1 isoform isolated from the sea bass gills and have identified, by immunofluorescence, NKCC1, and other isoforms, within the epithelium of the major osmoregulatory organs. Different (absorptive and secretory) functions have been attributed to this protein according to the tissue and salinity. The effects of short- (1-4 days), medium- (7-21 days) and long (6 months)-term freshwater (FW) adaptations were investigated, in comparison with seawater (SW)-maintained sea bass. In adult sea bass after... |
| Tipo: Text |
Palavras-chave: Quantitative expression; Immunofluorescence; Osmoregulation; Ontogeny; Dicentrarchus labrax; Na+/K+/2Cl( ) cotransporter. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-3531.pdf |
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